The success of the artificial breeding of the “Four Home Fish†is not only important for broodstock breeding, but also for supporting breeding equipment, and the strict and serious operation. The selection and use of oxytocin is also very important. Common oxytocins include salmon, salmon pituitary (PG), chorionic gonadotropin (HCG), luteinizing hormone-releasing hormone analogues (LRH-A), domoxone (DOM), reserpine (RES), etc. . The dose used is as follows (in terms of female body weight, the male fish is halved):
A single injection of the pituitary was used alone: ​​salmon, grass carp, carp used 3 to 4 mg per kilogram of body weight; black carp used 4 to 6 mg per kilogram of body weight. Secondary injection: The first needle of several kinds of fish is 0.3 mg per kilogram of body weight, the second needle carp, grass carp, carp weight of 3 to 4 mg per kilogram of body weight, herring weight of 4 to 6 mg per kilogram of body weight.
HCG alone is used to produce salmon and squid with one injection. The body weight per kilogram is 800 to 1200 IU.
Use LRH-A alone to produce a single injection of grass carp: 10 to 15 micrograms of body weight per kilogram of fish. Secondary injections: 1 μg for the first needle and 10 μg to 15 μg for the second needle.
HCG and LRH-A mixed use puerperal carp, carp: the first needle injection of 1-2 micrograms of LRH-A per kilogram of fish body weight, the second injection of 10 to 15 micrograms per kilogram of fish body weight LRH-A plus 300 ~ 400 IU HCG. Induced herring: The first needle was injected with 1-2 micrograms of LRH-A per kg of fish body weight, and the second needle was injected with 15-20 micrograms of LRH-A plus 800-1200 IU of HCG per kg of fish body weight.
In recent years, some units have used high-efficiency new-type fish oxytocin mixture to achieve better reproductive performance. "Four Home Fish" is commonly used as a new efficient anaesthesia mixture No. II and No. I, that is, LRH-A plus DOM, or LRH-A plus RES. The specific reference dosage (in terms of female body weight, the male fish is halved). Carp and salmon: 10 to 50 micrograms of LRH-A plus 3 to 5 milligrams of DOM per kilogram of body weight. Grass carp: Use 10 to 20 micrograms of LRH-A plus 3 to 5 milligrams of DOM per kilogram of body weight. Herring: The first needle was injected with 9-10 mg RES plus 50 μg LRH-A per kilogram of body weight, and the second needle was injected with 50 μg LRH-A per kg body weight.
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