PCR Cloning Overview  Â
Cloning technology is one of the core technologies of molecular biology experiments. DNA fragments obtained by PCR amplification usually need to be cloned into a plasmid vector for subsequent experiments such as sequencing, subcloning, expression, and the like. The TA cloning technology designed by Taq DNA polymerase to add a prominent A base at the 3'-end of the product makes PCR product cloning more convenient and efficient:
PCR primers do not require the design of restriction endonuclease recognition sequences;
The PCR product does not have to be subjected to enzymatic cleavage, end-filling or addition of a linker;
High copy vectors and universal primer sequences facilitate sequencing, amplification, and digestion.
GenStar currently offers two PCR cloning systems: an EZ-T vector for cloning a PCR product containing a 3'-end overhang A base and its corresponding rapid ligation kit, and for cloning without 3'- The EZ-Blunt blunt-end vector of the blunt-ended PCR product of the terminal base and its corresponding ligation reaction kit. At the same time, GenStar has developed a new EZ-Lyse clone rapid detection kit, which is convenient for researchers to quickly select the target clone in a short time for subsequent experiments.
GenStar® PCR Cloning and Related Product Selection Guide
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