The Russian "Izvestia" reported that scientists have discovered that Bacillus thuringiensis has multiple strains that can synthesize different crystal proteins. These proteins belong to endotoxins, but endotoxins that kill moths are often ineffective against beetles, and vice versa. To solve this problem, experts from the National Institute of Microbial Genetics and Breeding of the Russian Industrial Institute selected two strains of Bacillus thuringiensis, and their synthetic endotoxins have different insecticidal ranges.
Through the hybridization of the two bacilli, the researcher finally developed a new bacillus strain. Its unique DNA (DNA) can simultaneously direct the synthesis of its two “endorses†unique to the parent endotoxin. Laboratory tests have shown that the cysts and endotoxins of the new bacilli can kill potato beetles several times as much as the “parents†of the bacillus and can cause 58% of the death rate of the gypsy moth larvae that damage fruit trees. The death rate of exposure to new bacillus in larvae of Mediterranean sea larvae fed on cereals and beans was low, at 26%. In addition, the mortality rates of cotton aphid, western flower thrips and certain leafhoppers that endanger a variety of fruits and vegetables can reach 59%, 53%, and 91%, respectively, after encountering the new bacilli.
Tianwen experimental results showed that the preparation containing new bacillus can effectively kill several species of aphids and thrips that are harmful to cotton, tobacco and roses, and the effect of disinfestation can be comparable to that of the chemical pesticide “malathion†used, but it The side effects on warm-blooded animals and some beneficial insects are lower than that of "malathion." Some pests that are resistant to chemical pesticides are still unable to withstand this new formulation.
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Gellan gum is a water-soluble anionic polysaccharide produced by the bacterium Sphingomonas elodea (formerly Pseudomonas elodea). The gellan-producing bacterium was discovered and isolated by the former Kelco Division of Merck & Company, Inc. in 1978 from the lily plant tissue from a natural pond in Pennsylvania, USA. It was initially identified as a substitute gelling agent at significantly lower use level to replace agar in solid culture media for the growth of various microorganisms .Its initial commercial product with the trademark as "GELRITE" gellan gum, was subsequently identified as a suitable agar substitute as gelling agent in various clinical bacteriological media.
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