The count of mycoplasma is not like the general bacteria using the general turbidimetric method, because the growth of mycoplasma in liquid medium is clear and transparent, so the general turbidimetry cannot be used for the counting of mycoplasma! To use CCU (ie color change unit method)! The principle is to detect the relative concentration of mycoplasma by using the metabolic activity of mycoplasma in liquid medium as an indicator!
Specific practice: Take 12 sterile small test tubes, add 1.8 ml of mycoplasma liquid medium to each tube, then add 0. 2 ml of the test mycoplasma liquid in the tube, mix thoroughly, and then Pipette 0. 2 ml of the mixture into the second tube, and then analogy, until after a tube in the zui, and then placed in a 37-degree incubator to culture, the color of the zui after the change of a tube for the detection of mycoplasma CCU, such as When the color change occurs in the sixth tube, the CCU of the test mycoplasma is: the sixth power CCU/ml of 10!
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