Bovine Serum Albumin (BSA) is a globulin in bovine serum, also known as the fifth component. (Note that the fifth component is not a component of BSA, but Edwin J. Professor Cohn obtained five components from the blood through a specific separation technique in the early stage, the fifth of which is albumin), the CAS number is 9048-46-8, contains 583 amino acid residues, and the molecular weight is 66.430 kDa. The point is 4.7. Bovine serum albumin has a wide range of applications in biochemical experiments, such as blocking reagents in western blots. The albumin in the blood mainly functions to maintain osmotic pressure, pH buffering, carrier action and nutrition. In serum-free culture of animal cells, the addition of albumin serves physiological and mechanical protection and carrier action.
Fatty acid-free bovine serum albumin product and application 
Bovine serum albumin (no fatty acids), or fatty acid-free bovine serum albumin, or Fat Acid Free BSA, contains no or very small amounts of fatty acids, such as Equitech Bio's BSA, BAH66. BSA purified by conventional methods is difficult to effectively remove the fatty acids it binds. In addition to the specially purified BSA products, the range of fatty acids in commercially available conventional BSA reagents generally ranges from 0.2 to 1.5 moles of fatty acid per mole of BSA. However, some biomedical studies require the use of BSA without free fatty acids, otherwise BSA contains high concentrations of fatty acids that will affect cell culture, differentiation, metabolic reactions, biochemical assays, and the like. For example, ordinary BSA reagents cannot be used in the differentiation of 3T3-L1 adipocytes, otherwise the differentiation and cellular metabolic state will be affected; when measuring the concentration of free fatty acids released from cultured tissue cells, BSA containing no fatty acid must also be used, otherwise it is difficult to be accurate. Triglyceride hydrolysis and free fatty acid release were determined. Related products are as follows:
Bovine serum albumin fen, no fatty acids
Equitech-Bio's bovine serum albumin fen , a fatty acid-free product is an ultrapure powder purified using a patented heat treatment method. After special degreasing treatment, the fatty acid content is less than 0.002% (w/w), cost-effective and easy to dissolve. The fatty acid-free BSA can be used as a carrier protein for fat metabolism-related analysis, or can be combined with a specific fatty acid to form albumin, which can be used to enhance the performance of cell culture systems; if the presence of fat or fatty acid in the experiment affects hormones or This product is preferred when reading cholesterol; it can also be used as a substitute for certain fat and fatty acid analyses. Also available in larger sizes such as 5kg and 10kg, it is the highest quality and most cost-effective fatty acid-free BSA bovine serum albumin product on the market.
How to choose the right bovine serum albumin (BSA)?
Because bovine serum albumin (BSA) has a variety of product styles, it can meet different applications. At present, most of the information on the Internet is ambiguous, including bovine serum albumin and bovine serum albumin v. Many suppliers are unclear about the difference. In addition, there are no proteases, no DNase and no RNase on the market. There are no different BSA powders such as fatty acid, microbial grade, reagent grade, diagnostic analysis grade, etc. Therefore, how to choose the right BSA product is an important prerequisite. In general, ordinary BSA powder or fifth component is suitable for cell and tissue culture, and can also be used for some protein supplements; while other BSA without protease, DNase and RNase-free, fatty acid-free treatment is suitable for more A variety of applications in immunology, protein science, etc., as a blocking agent, protein Marker, hapten carrier and protein stabilizer. Here's a brief introduction:
| BSA type | Product features and applications |
| Standard grade BSA | The heat-treated ordinary BSA product can be used as a nutrient and culture component for tissue cells (microorganisms, animal and insect cells, etc.) and a protein molecular weight standard component, and is a cost-effective BSA product such as EquiTech-Bio's BAH62 series. |
| BSA fifth component | The fifth component of BSA is more pure than ordinary BSA. The fifth component of BSA is purified using the Cohn method and is a biotech grade product, generally used for cell culture, as a hapten carrier, a protective agent, and a protein supplement control. Such as EquiTech-Bio's BAC62 or BAC63 series products. |
| No IgG reagent grade BSA | The reagent grade BSA has better purity than the fifth component of BSA, which does not contain globulin IgG components that may interfere with the analysis. It can be used as a cell culture nutrient and culture component as well as a protein molecular weight standard component. Such as EquiTech-Bio's BAH64 series products. |
| Protease-free BSA | Protease-free bovine serum albumin can be used in cell culture to produce protein assays, particularly for expressing proteins that are sensitive to protease activity. In addition, protease-free bovine serum albumin is also very suitable. Such as EquiTech-Bio's BAH67 series BSA products and BAC65 series fifth component products. |
| Fatty acid free BSA | Fatty acid-free BSA can be used as a carrier protein for fat metabolism-related analysis, or combined with a specific fatty acid to form albumin, which can be used to enhance the performance of cell culture systems; if the presence of fat or fatty acids in the experiment affects hormones or cholesterol This product is preferred when reading. Such as EquiTech-Bio's BAH66 series. |
| Low endotoxin BSA | Low endotoxin bovine serum albumin is an ideal choice for cell and tissue culture. It can be used as a carrier for serum-free and synthetic media supplements. It is also used as a general reagent in the laboratory to stabilize and dilute sensitive protein solutions. It can also be used as a diagnostic and analytical tool such as ELISA. For example, EquiTech-Bio's BAH series has an endotoxin content of less than 1.0 EU/mg. |
| Protease-free/IgG BSA | It does not contain protease and RNase enzyme activity, and does not contain IgG. It has a higher purity grade and is used as a diluent or blocking solution in tests such as EIA and ELISA. Such as Jackson's 001-000-16X series and EquiTech-Bio's BAH67 (no protease / IgG, also does not contain RNase) series products. |
| Diagnostic BSA | Diagnostic grade bovine serum albumin fen is specially designed to reduce factors such as background interference and inhibition of cell culture systems, without proteases, bioburden and IgG, with very low activity of endotoxin (less than 3.0 EU/mg) . It can be used as a protein standard, diluent, conjugate or enzyme stabilizer, or as an immunoassay for highly sensitive immunoassay experiments, cell culture, and hybridization experiments. Such as EquiTech-Bio's BAH68 series. |
As a pioneer in global serum production processes and separation technologies, Equitech-Bio continues to lead the way in quality, assurance and economy, and has become the gold standard for major research institutions and pharmaceuticals worldwide. Equitech-Bio ultimately provides customers with optimal batch-to-batch stability through demanding inspections of every step of the product process and rigorous quality control procedures. Equitech-Bio's regional distributor in China, Ai Meijie Technology Co., Ltd. will provide you with professional technical advice, after-sales and product delivery services. We offer you the complete solution that's right for you by offering customized formulations and packaging options. Please contact Ai Meijie Technology Co., Ltd. for more product and technical solutions.
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