Detection of micrometastases in sentinel lymph nodes in breast cancer

Release date: 2007-12-10

Detection of micrometastasis in sentinel lymph nodes in breast cancer The best way to detect SLN micrometastasis is to have 100 μm intervals, continuous sections on two levels, and HE and mucl staining to detect the vast majority of micrometastases. Breast cancer is a disease in which the ductal epithelial cells of the breast undergo a variety of internal and external carcinogenic factors, and the cells lose their normal characteristics and become abnormally proliferating, resulting in cancerous changes beyond the limits of self-repair. In the second issue of the Chinese Journal of Oncology in February, a study was conducted in which the researchers used the isotope method to detect SLN in breast cancer. SLN, which was negative for routine pathology, was serially sliced ​​at intervals of 100 μm, and HE and immunohistochemistry were performed. Staining was used to detect micrometastasis; tumor specimens were taken for serial sectioning and immunohistochemical staining was performed in parallel. To explore the best method for detecting micrometastasis of sentinel lymph node (SLN) in breast cancer, and to study the correlation between clinicopathological factors and micrometastasis.
Research by Zhang Tao, from the Department of Abdominal Surgery, Cancer Hospital, Chinese Academy of Medical Sciences, Chinese Academy of Medical Sciences, showed 121 samples of SLN and 44 tumor specimens in 59 patients, and 17 (14.0%) of 14 patients (23.7%). SLN has a small transfer. With HE staining, when the number of slices was increased from 1 to 3, the number of detections of micrometastases was 3, 7, and 10, respectively; in three layers, the slices were successively sliced, and HE was separated from AE1/3 and CK19, respectively. When mucl was combined, the number of detections of micrometastases was 14, 12, and 16 cases, respectively. Increasing the number of sections or using a combined detection method can increase the number of micrometastases detected, and micrometastases are associated with primary tumor size, c-erbB2, MMP-2, and vascular endothelial growth factor (VEGF) expression. Therefore, it can be seen that the best method for detecting the small transfer of SLN is that the interval is 100 μm, and the slices are successively sliced ​​at two levels, and HE and mucl staining are performed simultaneously, and most of the micrometastases can be detected. ——Midi Medical Network

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