1. The broodstock choose to choose high-quality blue or yellow species, female body length 35 to 60 cm, weight 30 to 200 grams, there is a transparent zona pellucida, genital pores redness; see eggs better. Males selected more than 55 cm, weighing 150 to 450 grams, see blood spots on the ventral veins, abdominal pressure out of the transparent fluid, microscopic examination showed sperm. The ratio of male to female cockroaches is 2 to 3:1
2. Inflating urine production, increasing the amount of water exchange, adjusting the breeding density and providing high-quality bait, etc., to promote its development and improve natural reproduction. LRH-A can also be injected once in a place where conditions permit. Females were administrated at 10 micrograms per 50 grams, males at 15 to 25 micrograms per tail, and males were given intrapleural injections 24 hours later than females. When injected, the depth should not exceed 0.5 cm and the injection volume should not exceed 1 ml. After the injection of the intimacy, release it into a concrete pool or a cage and raise it to a depth of 20-30 cm. Change the water once a day. The water temperature is 25°C. After 36 hours of hand touching the abdomen, when the eggs are free, artificial insemination can be performed immediately.
3. Artificial insemination with one hand to hold the front of the intimacy with a towel, the other hand to squeeze the abdomen back, such as the cloacal blockage, scissors can be used to open the cloacal expansion, and then squeeze the egg. Place the eggs out of the cylinder or basin. Kill the selected males and take the test results of the testes. If the sperm activity is normal, cut the testes and grind them. Use a 100-mesh sieve to squeeze the semen into the egg container. Stir and mix for 5 to 7 minutes. Then use clean water. Wash away the testis residue and blood stains, and place the selected eggs in an incubator. Regardless of the size of the egg, insemination was performed using more than 2 males.
4. It is advisable to artificially incubate the incubation water to a depth of about 10 cm. Change the water frequently during incubation and the temperature difference should not exceed 1°C. Embryos consume different amounts of oxygen in different stages. For example, at 24°C, 100 oocytes consumed oxygen 0.29 mg/h, blastocyst 0.46 mg/h, and gastro-intestinal 0.53 mg/h. Later in the period, more oxygen was consumed, so conditions should be aerated. The incubation time is related to the water temperature. Within a suitable temperature range, the higher the water temperature, the less time is required, for example, 288 hours at 22°C, 192 hours at 23°C, and only 134 hours at 26°C.
5. It is recommended to use a small concrete pool for fry cultivation. The depth should be 30-40 cm. The upper edge should be 15-20 cm above the water surface. Add 5 centimeters to the bottom of the pool, press 0.5 to 1 kg/m2, apply manure, water and fertilizer, pluck silkworms, and stock aquatic plants such as water hyacinth. The cultivation density is 100 to 200 tails per square meter. It is better to use silkworms as the open bait, but it is also possible to feed zooplankton or fish paste, egg yolk powder, etc. Avoid large and small pools to avoid self-mutilation. Often add new water after 6 months can grow to 8 centimeters or so, at the end of the body 15 centimeters long, weighs about 39 grams, can develop into the pool.
2. Inflating urine production, increasing the amount of water exchange, adjusting the breeding density and providing high-quality bait, etc., to promote its development and improve natural reproduction. LRH-A can also be injected once in a place where conditions permit. Females were administrated at 10 micrograms per 50 grams, males at 15 to 25 micrograms per tail, and males were given intrapleural injections 24 hours later than females. When injected, the depth should not exceed 0.5 cm and the injection volume should not exceed 1 ml. After the injection of the intimacy, release it into a concrete pool or a cage and raise it to a depth of 20-30 cm. Change the water once a day. The water temperature is 25°C. After 36 hours of hand touching the abdomen, when the eggs are free, artificial insemination can be performed immediately.
3. Artificial insemination with one hand to hold the front of the intimacy with a towel, the other hand to squeeze the abdomen back, such as the cloacal blockage, scissors can be used to open the cloacal expansion, and then squeeze the egg. Place the eggs out of the cylinder or basin. Kill the selected males and take the test results of the testes. If the sperm activity is normal, cut the testes and grind them. Use a 100-mesh sieve to squeeze the semen into the egg container. Stir and mix for 5 to 7 minutes. Then use clean water. Wash away the testis residue and blood stains, and place the selected eggs in an incubator. Regardless of the size of the egg, insemination was performed using more than 2 males.
4. It is advisable to artificially incubate the incubation water to a depth of about 10 cm. Change the water frequently during incubation and the temperature difference should not exceed 1°C. Embryos consume different amounts of oxygen in different stages. For example, at 24°C, 100 oocytes consumed oxygen 0.29 mg/h, blastocyst 0.46 mg/h, and gastro-intestinal 0.53 mg/h. Later in the period, more oxygen was consumed, so conditions should be aerated. The incubation time is related to the water temperature. Within a suitable temperature range, the higher the water temperature, the less time is required, for example, 288 hours at 22°C, 192 hours at 23°C, and only 134 hours at 26°C.
5. It is recommended to use a small concrete pool for fry cultivation. The depth should be 30-40 cm. The upper edge should be 15-20 cm above the water surface. Add 5 centimeters to the bottom of the pool, press 0.5 to 1 kg/m2, apply manure, water and fertilizer, pluck silkworms, and stock aquatic plants such as water hyacinth. The cultivation density is 100 to 200 tails per square meter. It is better to use silkworms as the open bait, but it is also possible to feed zooplankton or fish paste, egg yolk powder, etc. Avoid large and small pools to avoid self-mutilation. Often add new water after 6 months can grow to 8 centimeters or so, at the end of the body 15 centimeters long, weighs about 39 grams, can develop into the pool.