Cell Transplant Application--CellMax® Hollow Fiber Cell Transplant Tube (Film) for Drug Screening

Cell transplantation application--CellMax ^® hollow fiber cell transplantation tube (membrane) for drug screening

In vivo screening of potential anti-cancer or anti-HIV active compounds is no longer common, and the National Cancer Institute has developed a new technology for screening anticancer drugs that transplants human cells into host animals and then remove them together. In the host, the cells are contacted with a potential chemotherapeutic agent, and the results of the action can be determined by taking the cells out. A well-compatibility hollow fiber cell transplant tube encapsulates a large number of cells and is transplanted into the subcutaneous or peritoneal cavity of laboratory rodents. After screening a portion of the compounds through a hollow fiber encapsulation test, the viability of these compounds can be confirmed using classical evaluation methods of current chemotherapeutic agents.
This method can significantly save time, manpower, compound dosage and number of experimental animals. Hollow fiber assay is the only subcutaneous or intraperitoneal in vivo model that can simultaneously test compounds affecting six cell lines (Hollingshead, et al. Life Sci. 57: 131, 1995), this model is used to determine the relationship between compound activity and cell density.
In addition, the model can also be used for mechanism of action studies, such as the Flavorpiriodal racemic mixture (YZ149, a literature standard and cyclin-dependent kinase inhibitor), and the density of intracellular NCT116 cells (human colon tumors) is 1.0, 5.0. Or 7.5 × 106 cells / mL, and then transplanted subcutaneously or intraperitoneally into nude mice. YZ149 was administered subcutaneously every day for 3 to 6 days after transplantation at doses of 6, 12, 18, and 25 mg/kg. MTT dye conversion assay can be used to determine cell growth status. When the cell density reaches 1 x 106 cells/mL, the number of cells no longer increases, indicating that the compound is active against higher cell density. After administration, cells were removed from the hollow fiber graft tube, and changes in expression of cell cycle-associated proteins such as SCK, cyclin and Rb were analyzed by Western Blot. The change in protein is the activity in vivo. The data demonstrates that the role of the compound is to affect cell density. This test shows that drug activity can be used to control cell density.

Hollow fiber cell transplantation tube (membrane)
Drug Development and Development The unique hollow fiber cell graft tube developed by CellMax® Hollow Fiber Cell Transplant Tube USA SPECTRUM provides realistic results for drug development and cancer research.
The modified polyvinylidene fluoride (mPVDF) hollow fiber material has good biocompatibility, anti-cell adhesion, hydrophobicity, and resistance to various organic solvents including most acid-base solutions.
The hollow fiber cell graft can be heat sealed, sterilized by high temperature and high pressure steam, and does not destroy the molecular retention of the membrane (MWCO 500,000D) without changing the properties of the membrane.
TRUE-to-LIFE! CellMax® hollow fiber cell grafts with “Teflon-like” surfaces can be retained in a variety of animal models for up to 14 days without causing rejection.
A variety of cell lines can be cultured in vitro or in vivo using CellMax® hollow fiber cell transplant tubes. After transplantation into animal hosts, the cell lines proliferate in the CellMax® hollow fiber graft without being attacked by the immune system. The use of CellMax® hollow fiber cell tubes encapsulating cancer cell lines into mice for screening for compounds having anticancer activity has been used. There are also compounds that screen for HIV-infected cells to screen for compounds that have anti-HIV activity.
Packageable cell types:
 Clear cancer cell line  Virus-infected cells  Hematopoietic stem cells  Bacteria  Fungi
CellMax® Hollow Fiber Cell Transplant Tubes Block:
 immune cells  virus  mycoplasma
CellMax® Hollow Fiber Cell Transplant Tubes have:
 Advantages of good biocompatibility of the inner and outer surfaces compared to current hybrid models:
 The experimental time is shortened from 60 days to 10 days.  The experimental error is smaller and the number of experimental animals is reduced.  The compound dosage is less.  One animal can simultaneously test multiple cell lines.  Suitable for different cell line specifications:
Packing: Wet type (high pressure steam sterilization) / dry type (not sterilized)
Material: PVDF
Inner diameter: 1.0mm
Outer diameter: 1.2mm
Length: 34cm / number of roots: 3 / package

Model: CellMax® Hollow Fiber Cell Transplant Tube (Film)

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